Research and Development (R & D) activities
In order to provide proper direction and to channelize future Pharma research and keeping the recent developments in mind the faculty members of various departments under the able guidance of Dr. B.K.Dubey are actively involved in thrust areas of research in different fields of Pharmacy such as; Advanced Drug Delivery Systems, Transdermal Systems, Gastroretentive and Nanoparticles; Pharmacological and Phytochemical screening of various indigeneous plants and Chemical synthesis of some Novel heterocyclic compounds of biological significance, Standardisation of Herbal formulations and recent advances in Pharmacy.
Following are the research works that are currently going on in the institution:-
- Preparation and Characterization of Lamivudine loaded Solid Lipid Nanoparticles for the treatment of AIDS.
- Green synthesis of Nano-Silver particles for its Anti-bacterial activity.
- Design and Evaluation of Transdermal delivery of Ketoprofen via Noisome entrapped in Bio-adhesive Gel system.
- Design and Characterization of Metformin loaded Microballoons: An approach to controlled drug delivery via gastric retention.
- Solubility enhancement of a poorly aq. soluble drug by using Mixed Solvency Technique.
- Development and Evaluation of Ondansetron floating tablets.
- Design and Evaluation of Transdermal patches of Valsartan.
- Preparation and Evaluation of Mucoadhesive Microspheres of Simvastatin.
- Development and Characterization of Lamivudine sustained release matrix tablets.
Research Works Presented By M.Pharm Students In Seminars
- Mohit Sahu won First prize in Presentation in the National seminar organized by NRI Institute of Pharmacy,Bhopal in the year 2009. The Research paper presented by the student-
- “Design And Evaluation Of Monolithic Matrix Type Transdermal Patches Using Non-Antigenic And Biocompatible Polymer.”
Abstract
Transdermal drug delivery systems (TDDS) allow delivery of contained drug into the systemic circulation via permeation through skin layers at a controlled rate while bypassing first pass metabolism has accelerated Transdermal drug delivery research in the field of pharmaceutics. These systems are easy to apply and remove as and when desired. This approach of drug delivery is more pertinent in case of chronic disorders, such as hypertension, which require long term dosing to maintain therapeutic drug concentration.
One of the major limitations of TDDS is that sometimes it may induce an irritation or sensitization reaction of the skin. These skin reactions may be elicited by the drug itself or by the materials used to fabricate the devices. Patch testing of some TDDSs has revealed that some of these skin reactions are directed against the device and are not due to drugs. One probable approach to minimize the device associated adverse skin reactions of TDSs is to use highly biocompatible and non-antigenic biopolymers such as chitosan for their fabrication. In the present investigation Monolithic matrix type transdermal drug delivery systems of Atenolol was prepared using the natural polymer, chitosan. Atenolol is a β1 blocker, incompletely absorbed from GIT and the mean elimination half-life is 6-7 hours. The bioavailability of the drug is 40-50%. Atenolol is used to treat cardiovascular diseases and conditions such as hypertension, coronary heart disease, arrhythmias, angina and to treat and reduce the risk of heart complications following myocardial infarction.
The aim of this work is to investigate the possibility of obtaining a prolonged, relatively constant effective level of Atenolol from transdermal drug delivery system using natural, biocompatible polymer chitosan. This type of matrix TDDS was prepared by using Solvent Evaporation technique. Characterisation of polymeric films were performed by evaluating factors such as thickness uniformity, Folding endurance, Moisture uptake, Weight variation, Drug content uniformity, skin irritation test, drug polymer interaction by FTIR, In-vitro permeation studies of drug loaded patch in modified Franz diffusion cell. From the IR studies it is seen that there is no interaction between drug and polymer, which shows that drug is compatible with polymer. Kinetic study was performed and the formulation showed zero order release by diffusion mechanisim. Further work is necessary to study in vivo release characteristics.
- Vivek Bhowsar won First prize in Presentation in the National seminar on Advance Approaches in Drug Designing and Drug Targetting, organized by GRY Institute of Pharmacy, Vidya Vihar, Borawan in the year 2009.
The Research paper presented by the student-
“Formulation and Characterization of Niosomes for Ocular delivery of Indomethacin for treatment of Uveitis.”
- Sonam Jain won First prize in Presentation in the National seminar organized by Laxmi Narayan College of Pharmacy, Bhopal in the year 2009. The Research paper presented by the student-
“Design and Characterisation of Polymethacrylate Microspheres of Ketoprofen by spherical crystallization technique.”
Abstract
Microspheres which are matrix systems containing the drug throughout the structure are potential candidates for oral controlled release. Methacrylate copolymers (Eudragit RS) have received increased attention for preparing sustained dosage forms because of their inertness, solubility in relatively non-toxic solvents and availability of resins with different properties.
Present investigation describes the formulation and development of sustained drug delivery system of Ketoprofen. Polymethacrylate microspheres containing Ketoprofen were prepared in four different drug to carrier ratio F1 (1:0.5), F2 (1:1), F3 (1:2) and F4 (1:3) by using spherical crystallization technique. The prepared microspheres were characterized for particle size, particle shape, flow property, percentage yield, drug entrapment, stability studies, In vitro drug release and kinetic study.
The shape of microspheres was found to be spherical by scanning electron microscope. FT-IR study confirmed that there was no interaction between drug and polymers. The size of microspheres was found to be ranging 48.3 µm to 246.4 µm. All formulation showed good flow property in terms of angle of repose. Percentage yield and entrapment efficiency was in the range of 78.12 – 86.25 % and 83.21 – 94.36 % w/w respectively. No appreciable difference was observed in the extent of degradation of product during 60 days in the microspheres which were stored at various temperatures. In the In-vitro drug release study F1, F2, F3 and F4 showed 92.3, 80.5, 67.8 and 58.8% drug release at 12 hours and found to be sustained.
- Vivek Bhowsar won second prize in Presentation in the conference Pharma Meet-2009 organized by Indian Pharmaceutical Association (IPA), Indore. The Research paper presented by the student –
- “Formulation and Evaluation of Floating Drug Delivery System of Lansoprazole.”
Abstract
Microparticulate floating dosage forms provide us with new and important therapeutic options to improve the release profile of the drug and also target it to stomach and upper intestine via gastric retention. In the present study, Floating microspheres containing Lansoprazole was prepared by emulsion solvent diffusion method using various ratios of Eudragit RS 100 (ES) and hydroxypropylmethylcellulose (HPMC). The polymer content was a mixture of EC:HPMC, 10:0(F1),9:1(F2),8:2(F3) and 7:3(F4). The Prepared floating Microspheres were characterized for particle size, shape, flow properties, bulk density, true density, compressibility, porosity, percentage yield, drug entrapment, in vitro floating behavior and for in vitro drug release. The microspheres were found regular in shape by scanning electron microscopy. All floating microparticles formulations showed good flow properties and packability. Percentage entrapment was decreasing on increasing the concentration of HPMC. FT-IR study confirmed that there was no interaction between drug and polymers and stability of drug during formulation. The floating ability was tested in 0.1 N HCL containing 0.02 % Tween. The drug release rate was tested in 0.1 N HCL (pH 1.2) containing Tween 20 (0.02% w/v) or phosphate buffer pH 6.8 containing Tween 80 (0.05% w/v). The formulation containing EC:HPMC (9:1) demonstrated favorable in vitro floating and in vitro release rate characteristics.
- Neha Rao won Third prize in Presentation in the seminar organized by TIT College of Pharmacy, Bhopal in the year 2009. The Research paper presented by the student –
“Design And Characterization Of Dipyridamole Loaded Microspheres: An Approach To Controlled Drug Delivery Via Gastric Retention.”
Abstract
Gastroretentive floating microspheres provide us with new and important therapeutic options to improve the release profile of the drug and also target it to stomach and upper intestine via gastric retention. In the present study, Floating microspheres containing dipyridamole was prepared by emulsion solvent diffusion method using various ratios of Eudragit RS 100 (ES) and hydroxypropylmethylcellulose (HPMC). Dipyridamole is a poorly-soluble base, dissolves rapidly in stomach but incompletely in intestine and absorption is remarkably lower in elevated gastric pH making it an ideal candidate for floating microballons. The polymer content was a mixture of EC:HPMC, 10:0(F1),9:1(F2),8:2(F3) and 7:3(F4). The Prepared floating microspheres were characterized for particle size, shape, flow properties, bulk density, true density, compressibility, porosity, percentage yield, drug entrapment, in vitro floating behavior and for in vitro drug release. The microspheres were found regular in shape,spherical and hollow within the sphere by scanning electron microscopy. All floating microspheres formulations showed good flow properties and packability. Percentage entrapment was decreasing on increasing the concentration of HPMC. FT-IR study confirmed that there was no interaction between drug and polymers and stability of drug during formulation. The floating ability was tested in 0.1 N HCL containing 0.02 % Tween. The drug release rate was tested in 0.1 N HCL (pH 1.2) containing Tween 20 (0.02% w/v) or phosphate buffer pH 6.8 containing Tween 80 (0.05% w/v). The formulation F2 containing EC:HPMC (9:1) demonstrated favorable in vitro floating and in vitro release rate characteristics.
- Vishnu Sharma won third prize in poster presentation in national level seminar organized by NRI Int. of Pharm. Sciences, Bhopal in 2009.
Topic of Presentation: “Formulation & Evaluation of Lamivudine Sustained released Matrix Tablets”
Abstract
The study was undertaken with an aim to formulation development and evaluation of Lamivudine sustained release tablets using polymers hydroxypropylmethylcellulose and ethylcellulose. Lactose monohydrate was used as channeling agent and or as filler. Preformulation study was done initially and results directed for the further course of formulation. Based on preformulation studies different formulations of Lamivudine were prepared using selected excipients. FT-IR study performed for the identification and compatibility study of drug with polymers and found the characteristics peaks of various groups and matched with pharmacopoeial standard. Powder and blends were evaluated for tests - bulk density, tapped density, compressibility index, Hausner’s ratio before being punched as tablets. Tablets were prepared by direct compression method by using 9 station single rotary tablet compression machine with application of 6-7 tons pressure. After in process evaluations (discussed below) tablets are packed in well closed moisture proof, light resistance container, labeled, and kept at dry place. Tablets were tested for official and unofficial tests like- weight variation test, thickness, hardness, friability and In vitro drug release as per official procedure was performed to observe diffusion and release mechanism of drug through polymeric membrane.
- Students participated and presented Research papers in the National seminar on Frontiers in Nanotechnology: A Pharmaceutical Perspective, organized by Nirma University, Institute of Pharmacy, Ahmedabad, Gujrat. Following are the Research papers presented by students -
- “Design and Evaluation of Non-ionic Surfactant based vesicles for Ocular delivery of Gatifloxacin.”
- “Preparation and Evaluation of Polymethacrylate Microspheres of Metformin by Spherical Crystallization.”
- “Development and Evaluation of Ketoprofen loaded floating oral delivery system.”
List of Publication in Journals
Accepted Articles
- “Study of formulation Variables influencing the drug release rate from Matrix tablets by Experimental Design.” is accepted and shall be published in the ‘International Journal of Pharmaceutical Sciences and Technology.’
AUTHOR : G. K. PANDEY, MITHUN BHOWMICK, B.K. DUBEY
- “Development and evaluation of Lamivudine Sustained Release Matrix Tablets.” is accepted and shall be published in ‘International Journal of Pharm Tech Research.’
AUTHOR : G. K.PANDEY, B.K. DUBEY
- “Design and evaluation of Non-ionic surfactant based Niosomes for Ocular delivery of Indomethacin for the treatment of Uveitis.” is accepted and shall be published in ‘Research Journal of Pharmacy and Technology.’ AUTHOR : MITHUN BHOWMICK, B.K. DUBEY
- “Synthesis, Characterization and In-Vitro Cytotoxic activity of some novel 2-substituted amine-4-dimethylamine-6-(n-phenyl-1-naphthylamino)-1,3,5-triazine” is accepted and shall be published in ‘The Young Pharmacist.’ AUTHOR : DEEPAK BASEDIA, B.K. DUBEY
- “Preliminary Phytochemical Screening of Tephrosia Purpurea of family Leguminoceae” is accepted and shall be published in ‘Research Journal of Pharmacognosy and Phytochemistry.’ AUTHOR : RAMANUJ PATEL, B.K. DUBEY
- “Antimicrobial Activity of Terminalia Balerica” is accepted and shall be published in the ‘International Journal of Pharmaceutical Sciences and Technology.’
AUTHOR : NARENDRA PRAJAPATI, B.K. DUBEY
- “Synthesis, Characterization and Anti-Microbial activity of some schiff’s bases of Novel 2-sustituted Amino-n-(-2-Flourophenyl)-4,5- Dimethyl Thiophenes-3-Carbaxamide is accepted and shall be published in the ‘International Journal of Chem tech Research.’
AUTHOR : DEEPAK BASEDIA, B.K. DUBEY
- “The scope of Web-Based Clinical Research in Pharmacy” is accepted and published in ‘Research Journal of Pharmacy and Technology.’
AUTHOR : G. K. PANDEY
- Application of DNA Based Markers in Molecular Genetics: An Update is accepted and shall be published in the ‘International Journal of Pharmaceutical sciences and technology.’
AUTHOR : SAURAV RAJVEDYA, B.K. DUBEY
- “Design And Evaluation of Monolithic Matrix Type Transdermal Patches Using Non-Antigenic and Biocompatible Polymer” is accepted and shall be published in the ‘International Journal of Pharm Tech Research.’
AUTHOR : MOHIT SAHU, B.K. DUBEY
- “Preparation and Characterization of Lamivudine loaded Solid Lipid Nanoparticles for the treatment of AIDS.” is accepted and shall be published in the ‘International Journal of Pharm Tech Research.’
AUTHOR : NEHA RAO, B.K. DUBEY